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內(nèi)容簡介

　　本書包括20個(gè)再生藥物及傳遞常用實(shí)驗(yàn)項(xiàng)目，首先介紹了再生醫(yī)學(xué)的實(shí)驗(yàn)場地要求，其次介紹了組織工程構(gòu)建時(shí)種子細(xì)胞的分離和培養(yǎng)，重點(diǎn)介紹原代細(xì)胞分離、純化和培養(yǎng)的方法，之后重點(diǎn)介紹了幾種常用的藥物傳遞系統(tǒng)的構(gòu)建，包括納米粒、脂質(zhì)體、微囊等，同時(shí)介紹了幾種常用的組織工程支架構(gòu)建方法，此外，結(jié)合 3D打印技術(shù)，介紹同軸打印的方法構(gòu)建組織工程化血管，介紹了幾種常見再生藥物及傳遞的儀器使用方法。本書的出版能幫助留學(xué)生好地進(jìn)行再生醫(yī)學(xué)和藥物、基因輸送相關(guān)實(shí)驗(yàn)，對(duì)提高留學(xué)生教學(xué)質(zhì)量及來華留學(xué)生課程建設(shè)具有積極意義。

作者簡介

暫缺《再生醫(yī)學(xué)和藥物、基因輸送實(shí)驗(yàn)手冊(cè)》作者簡介

圖書目錄

Chapter 1 Establishment of Tissue Engineering Laboratory
Laboratory design for cell culture
Rules and regulations for cell culture room
Chapter 2 Seed Cell Culture and Identification
Basic principles of in vitro cell culture
Experiment 1 Isolation and culture assay of primary cells
Experiment 2 Primary cell subculture and its passage assay
Experiment 3 Freezing and resuscitation of cells
Experiment 4 MTT method to measure cell proliferation rate
Experiment 5 Immunostaining assay
Experiment 6 DAPI nuclear fluorescent labeling assay
Experiment 7 Cell migration assay
Experiment 8 SDS-polyacrylamide gel electrophoresis assay
Experiment 9 Isolation and cultivation of rat bone marrow mesenchymal stem cells
Chapter 3 Fabrication of Three-dimensional Scaffolds
Experiment 10 Solvent casting assay
Experiment 11 Gel pressing method assay
Experiment 12 Preparation of injectable thermosensitive hydrogel
Experiment 13 Freeze-drying method assay
Experiment 14 Three-dimensional tissue printing technique assay
Experiment 15 Coaxial bioprinting of dual network bioink to prepare blood vessels
Chapter 4 Growth Factor Delivery Systems for Regenerative Medicine
Experiment 16 Preparation of hydrogel for growth factor delivery
Experiment 17 Application of hydrophobic polymers in GF transport
Experiment 18 Growth factor perfusion delivery assay
Experiment 19 pH sensitive drug delivery assay
Experiment 20 Magnetically responsive drug delivery assay
Chapter 5 Use of Common Instruments and Equipment for Tissue
Engineering
Workbench
Inverted fluorescence microscope
Carbon dioxide (CO2) incubator
Bioreactor
Laser confocal microscopy
References